Escherichia coli str. K-12 substr. MG1655 (ecol0)
Gene : murF
DDBJ :murF UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase
Swiss-Prot:MURF_ECOLI RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; EC=6.3.2.10;AltName: Full=UDP-MurNAc-pentapeptide synthetase;AltName: Full=D-alanyl-D-alanine-adding enzyme;
Homologs Archaea 0/68 : Bacteria 845/915 : Eukaryota 3/199 : Viruses 0/175 --->[See Alignment]
c.59.1
c.72.2
c.98.1
:452 amino acids
:SECSTR
:PSIPRED
:DISOPRED
:BLT:PDB 2->447 1gg4A PDBj 0.0 98.1 %
:RPS:PDB 8->443 1e8cA PDBj 2e-68 17.5 %
:RPS:SCOP 2->98 1gg4A3 c.98.1.1 * 1e-39 100.0 %
:RPS:SCOP 99->312 1gg4A4 c.72.2.1 * 2e-48 89.8 %
:RPS:SCOP 313->447 1gg4A1 c.59.1.1 * 1e-24 97.0 %
:HMM:SCOP 1->98 1gg4A3 c.98.1.1 * 4.2e-30 46.9 %
:HMM:SCOP 99->312 1gg4A4 c.72.2.1 * 9.5e-60 39.7 %
:HMM:SCOP 313->447 1gg4A1 c.59.1.1 * 6e-41 45.9 %
:RPS:PFM 27->95 PF01225 * Mur_ligase 2e-09 50.0 %
:RPS:PFM 105->283 PF08245 * Mur_ligase_M 2e-22 39.4 %
:HMM:PFM 106->292 PF08245 * Mur_ligase_M 4e-52 39.1 184/188
:HMM:PFM 313->394 PF02875 * Mur_ligase_C 2.6e-18 32.5 80/91
:HMM:PFM 25->94 PF01225 * Mur_ligase 3.2e-18 37.1 70/76
:BLT:SWISS 1->452 MURF_ECOLI 0.0 100.0 %
:SEG
SeqInfo AminoSeq See neighboring genes
Links GIB DAD PEC GenoBase Abbreviations Back to title page
GT:ID AAC73197.1
GT:GENE murF
GT:PRODUCT UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase
GT:DATABASE GIB00009CH01
GT:ORG ecol0
GB:ACCESSION GIB00009CH01
GB:LOCATION 94650..96008
GB:FROM 94650
GB:TO 96008
GB:DIRECTION +
GB:GENE murF
GB:PRODUCT UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase
GB:FUNCTION enzyme; Murein sacculus, peptidoglycan 1.6.7 metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein) 1.7.34 metabolism; central intermediary metabolism; peptidoglycan (murein) 1.7.34 metabolism; central intermediary metabolism; peptidoglycan (murein) turnover, recycling 7.1 location of gene products; cytoplasm
GB:NOTE GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0000270 - peptidoglycan metabolic process
GB:PROTEIN_ID AAC73197.1
GB:DB_XREF GI:1786274 ASAP:ABE-0000313 UniProtKB/Swiss-Prot:P11880 EcoGene:EG10622
GB:GENE:GENE murF
LENGTH 452
SQ:AASEQ MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALKGERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARVVALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELGANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMNADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLPLPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYNANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGKQSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENGTC
GT:EXON 1|1-452:0|
SW:ID MURF_ECOLI
SW:DE RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; EC=6.3.2.10;AltName: Full=UDP-MurNAc-pentapeptide synthetase;AltName: Full=D-alanyl-D-alanine-adding enzyme;
SW:GN Name=murF; Synonyms=mra; OrderedLocusNames=b0086, JW0084;
SW:KW 3D-structure; ATP-binding; Cell cycle; Cell division; Cell shape;Cell wall biogenesis/degradation; Complete proteome; Cytoplasm;Direct protein sequencing; Ligase; Nucleotide-binding;Peptidoglycan synthesis.
SW:EXACT T
SW:FUNC +
BL:SWS:NREP 1
BL:SWS:REP 1->452|MURF_ECOLI|0.0|100.0|452/452|
GO:SWS:NREP 9
GO:SWS GO:0005524|"GO:ATP binding"|ATP-binding|
GO:SWS GO:0007049|"GO:cell cycle"|Cell cycle|
GO:SWS GO:0051301|"GO:cell division"|Cell division|
GO:SWS GO:0008360|"GO:regulation of cell shape"|Cell shape|
GO:SWS GO:0007047|"GO:cellular cell wall organization"|Cell wall biogenesis/degradation|
GO:SWS GO:0005737|"GO:cytoplasm"|Cytoplasm|
GO:SWS GO:0016874|"GO:ligase activity"|Ligase|
GO:SWS GO:0000166|"GO:nucleotide binding"|Nucleotide-binding|
GO:SWS GO:0009252|"GO:peptidoglycan biosynthetic process"|Peptidoglycan synthesis|
SEG 178->187|aalvnnlaaa|
SEG 284->292|analaaaal|
BL:PDB:NREP 1
BL:PDB:REP 2->447|1gg4A|0.0|98.1|430/430|
RP:PDB:NREP 1
RP:PDB:REP 8->443|1e8cA|2e-68|17.5|423/482|
RP:PFM:NREP 2
RP:PFM:REP 27->95|PF01225|2e-09|50.0|68/76|Mur_ligase|
RP:PFM:REP 105->283|PF08245|2e-22|39.4|175/187|Mur_ligase_M|
HM:PFM:NREP 3
HM:PFM:REP 106->292|PF08245|4e-52|39.1|184/188|Mur_ligase_M|
HM:PFM:REP 313->394|PF02875|2.6e-18|32.5|80/91|Mur_ligase_C|
HM:PFM:REP 25->94|PF01225|3.2e-18|37.1|70/76|Mur_ligase|
GO:PFM:NREP 4
GO:PFM GO:0005524|"GO:ATP binding"|PF01225|IPR000713|
GO:PFM GO:0009058|"GO:biosynthetic process"|PF01225|IPR000713|
GO:PFM GO:0005524|"GO:ATP binding"|PF08245|IPR013221|
GO:PFM GO:0009058|"GO:biosynthetic process"|PF08245|IPR013221|
RP:SCP:NREP 3
RP:SCP:REP 2->98|1gg4A3|1e-39|100.0|97/98|c.98.1.1|
RP:SCP:REP 99->312|1gg4A4|2e-48|89.8|206/206|c.72.2.1|
RP:SCP:REP 313->447|1gg4A1|1e-24|97.0|135/135|c.59.1.1|
HM:SCP:REP 1->98|1gg4A3|4.2e-30|46.9|98/98|c.98.1.1|1/1|MurE/MurF N-terminal domain|
HM:SCP:REP 99->312|1gg4A4|9.5e-60|39.7|214/214|c.72.2.1|1/1|MurD-like peptide ligases, catalytic domain|
HM:SCP:REP 313->447|1gg4A1|6e-41|45.9|135/135|c.59.1.1|1/1|MurD-like peptide ligases, peptide-binding domain|
OP:NHOMO 1024
OP:NHOMOORG 848
OP:PATTERN -------------------------------------------------------------------- 1212211111111111111-11--11111111121211111--221111111111111111111111111111111111111122111222211221--112211323121111111111111121111111111122222---211111111111121211111112112111111111111111111111122232312312231122122222232115233222222221111111111111113222111111111111221111111111111111111111111111111111111111111111111111111111422211111111111111211111212211212221121111222111211-211211211211111111111122212122222-111111111112211211211111211222221111111111111111112111211------1-21111111111111111111111111111111111111111111111111111111111111111111111111111111211221111113111111111111111111111111-12111111111-----------1111---1-1---1111222112222111111212222112211211-11111-1-1-111111111111111111-11111111111111111111111111111111111111111111111111111111111111111111122222111112122111111111111122111111211111111111111111121111111111112222211111112111111111111111111211111111111111111--------------------------23-2112111111 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------1------2-----1------- -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
STR:NPRED 446
STR:RPRED 98.7
SQ:SECSTR #ccccHHcHHHHHTTTcTTccccccccEccGGGccTTcEEEEcccccccGGGGHHHHHHTTTTTccTTcEEETTEEEEEETTHHHHHHHHHHHHTTcGGGcHHHHHHHTTccEEEETTEEEETTcccEcccccccccHHHHHHHHHHHHHTTccEEEEccccHHHHHTTTTTTccccEEEEcccccccHHHHccHHHHHHHHHHHTTTcccccEEEEETTcHHHHHHHTTcTTcEEEEccTTTccEEEEEEEEEEccccEEEEEEETTcccEEEEEcccHHHHHHHHHHHHHHHHTTccHHHHHHHGGGccccTTcEEEccTTccEEEEEEcccccHHHHHHHHHHHHHTccccEEEEccccccccTHHHHHHHHHHHccEEEEccccccTccHHHHHHHHHTTcEEccHHHHHHHHHHcccTcTTcEEEEEccTTccEEEETHHHH#####
DISOP:02AL 452-453|
PSIPRED cccccHHHHHHHcccEEEccccccccEEcHHHHcccccEEEEccccccccHHHHHHHHHcccEEEEEcccccccccEEEEccHHHHHHHHHHHHcccccccEEEEEcccccHHHHHHHHHHHHHccccEEEEccccccccHHHHHHHHcccccEEEEEEEcccHHHHHHHHccccccEEEEccccHHHHHHcccHHHHHHHHHHHHHccccccEEEEccccHHHHHHHHHHccccEEEEccccccccEEEEEEEEcccEEEEEEEEcccEEEEEcccccHHHHHHHHHHHHHHHHccccHHHHHHHHHHcccccccEEEEEcccEEEEEEccccccHHHHHHHHHHHHHccccEEEEEEccccccccHHHHHHHHHHHHHHccccEEEEEcccHHHHHHHccccEEEccHHHHHHHHHHHccccccEEEEEccccccHHHHHHHHHHHcccc
//
[GTOP]
